Macho Lab
Molecular Plant-Bacteria Interactions
Shanghai Center for Plant Stress Biology
Publications
2021
Wang Y., Zhao A., Morcillo R.J.L., Yu G., Xue H., Rufian, J.S., Sang Y., and Macho A.P. A bacterial effector uncovers a novel pathway involved in tolerance to bacterial wilt disease. Molecular Plant (2021) 30:S1674-2052(21)00159-3. https://doi.org/10.1016/j.molp.2021.04.014
Rufian J.S., Rueda-Blanco J., Lopez-Marquez D., Macho A.P., Beuzon C.R., and Ruiz-Albert J. The bacterial effector HopZ1a acetylates MKK7 to suppress plant defense responses in Arabidopsis. New Phytologist (2021) https://doi.org/10.1111/nph.17442
Ruiz-Lopez N., Pérez-Sancho J., Esteban Del Valle A., Haslam R.P., Vanneste S., Catalá R., Perea-Resa C., Van Damme D., García-Hernández S., Albert A, Vallarino J., Lin J., Friml J., Macho A.P., Salinas J., Rosado A., Napier J.A., Amorim-Silva V., and Botella M.A. Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress. Plant Cell (2021) 4:koab122. https://doi.org/10.1093/plcell/koab122
Li Z., Luo X., Ou Y., Jiao H., Peng L., Fu X., Macho A.P., Liu R., and He Y. JASMONATE-ZIM DOMAIN proteins engage Ploycomb chromatin modifiers to negatively modulate Jasmonate signalling in Arabidopsis. Molecular Plant (2021) 14(5):732-747. https://doi.org/10.1016/j.molp.2021.03.001
de Pedro-Jove R., Sebastia P., Puigvert M., Macho A.P., Monteiro J.S., Coll N.S., Setubal J.C., and Valls M. Ralstonia solanacearum gene expression profiling at different potato infection stages. BMC Genomics (2021) 22(1):170. https://doi.org/10.1186/s12864-021-07457-w
Yu G., Xian L., Zhuang H., and Macho A.P. SGT1 is not required for bacterial PAMP-triggered immunity. Molecular Plant Pathology (2021) 22(1):145-150. https://doi.org/10.1111/mpp.13012
Ho Plagaro T., Morcillo R., Tamayo-Navarrete M.I., Huertas-Ruz R., Molinero-Rosales N., Lopez-Raez J.A., Haidour A., Macho A.P., and Garcia-Garrido J.M. DWARF14‐LIKE2 regulates arbuscule hyphal branching during arbuscular mycorrhizal symbiosis. New Phytologist (2021) 229(1):548-562. https://doi.org/10.1111/nph.16938
2020
Yu G., Xian L., Xue H., Yu W., Rufian J., Sang Y., Morcillo R., Wang Y., and Macho A.P. A bacterial effector protein prevents MAPK-mediated phosphorylation of SGT1 to suppress plant immunity. PLoS Pathogens (2020) 16(9): e1008933. https://doi.org/10.1371/journal.ppat.1008933
Xian L.#, Yu G.#, Wei Y., Rufian J.S., Li Y., Zhuang H., Xue H., Morcillo R.J.L., and Macho A.P. A bacterial effector protein hijacks plant metabolism to support pathogen nutrition. Cell Host & Microbe (2020), 28:548-557.e7. https://doi.org/10.1016/j.chom.2020.07.003
Wei Y., Balaceanu A., Rufian J.S., Segonzac C., Zhao A., Morcillo R., and Macho A.P. An immune receptor complex evolved in soybean to perceive a polymorphic bacterial flagellin. Nature Communications (2020) 11:3763 https://doi.org/10.1038/s41467-020-17573-y
Hao X, Lozano-Duran R.*, and Macho A.P.* Insights Into the Root Invasion by the Plant Pathogenic Bacterium Ralstonia solanacearum. Plants (2020) 9, 516.
Wang X., Ren M., Liu D., Zhang D., Zhang C., Lang Z., Macho A.P.*, Zhang M.,* and Zhu J.K.* Large-scale eQTL identification in Arabidopsis reveals novel candidate regulators of immune responses and other processes. Journal of Integrative Plant Biology (2020), online early https://doi.org/10.1111/jipb.12930 (* Co-corresponding authors)
Lee E., Santana B.V.N., Samuels E., Benitez-Fuente F., Corsi E., Botella M.A., Perez-Sancho J., Vanneste S., Friml J., Macho A.P., Alves-Azevedo A., and Rosado A. Rare Earth Elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes. Journal of Experimental Botany (2020) 71:3986–3998, https://doi.org/10.1093/jxb/eraa138.
Morcillo R., Zhao A., Tamayo-Navarrete M.I., García-Garrido J.M., and Macho A.P. A versatile method for tomato root transformation followed by inoculation with Ralstonia solanacearum allows straightforward genetic analysis for the study of bacterial wilt disease. Journal of Visualized Experiments (2020) 157, e60302. Link. Click here to watch the video article.
Sang Y., Yu W., Zhuang H., Wei Y., Derevnina L., Yu G., Luo J., and Macho A.P. Intra-strain elicitation and suppression of plant immunity by Ralstonia solanacearum type-III effectors in Nicotiana benthamiana. Plant Communications (2020) online early https://doi.org/10.1016/j.xplc.2020.100025
Wang P., Hsu C-C., Du Y., Zhu P., Zhao C., Fu X., Zhang C., Paez J.S., Macho A.P., Tao W.A., and Zhu J-K. Mapping Proteome-Wide Targets of Protein Kinases in Plant Stress Responses. PNAS (2020) 117:3270-3280. https://doi.org/10.1073/pnas.1919901117
Morcillo RJL., Singh SK., He D., An G., Vilchez JI., Tang K., Yuan F., Sun Y., Shao C., Zhang S., Yang Y., Liu X., Dang Y., Wang W., Gao J., Huang W., Lei M., Song C-P., Zhu J-K., Macho AP., Pare PW., and Zhang H. Diacetyl determines plant-bacteria relation via phosphate-dependent modulation of plant immunity. EMBO Journal (2020) 39:e102602. https://doi.org/10.15252/embj.2019102602. Link
2019
Garnelo-Gomez B., Zhang D., Rosas-Diaz T., Wei Y., Macho A.P., and Lozano-Duran R. The C4 protein from Tomato yellow leaf curl virus can broadly interact with plant receptor-like kinases. Viruses (2019) 31;11. https://doi.org/10.3390/v11111009. Link
Rubio L., Diaz-Garcia J., Amorim-Silva V., Macho A.P., Botella M. A., and Fernandez J.A. ZosmaNRT2 encodes the putative sodium dependent high-affinity nitrate transporter of Zostera marina L. International Journal of Molecular Sciences (2019) https://doi.org/10.3390/ijms20153650. Link
Sabbagh C. R. R., Carrère S., Lonjon F., Vailleau F., Macho A.P., Genin S., and Peeters N. Pangenomic type III effector database of the plant pathogenic Ralstonia spp. PeerJ (2019) 7:e7346 https://doi.org/10.7717/peerj.7346. Link
Duan J., Lee K. P., Dogra V., Zhang S., Liu K., Caceres-Moreno C., Lv S., Xing W., Kato Y., Sakamoto W., Liu R., Macho A. P. and Kim C. Impaired PSII proteostasis promotes retrograde signaling via salicylic acid. Plant Physiology (2019) doi: 10.1104/pp.19.00483. Link
Amorim-Silva V., García-Moreno A., Castillo A.G., Lakhssassi N., Esteban del Valle A., Pérez-Sancho J., Li Y., Posé D., Pérez-Rodriguez J., Lin J., Valpuesta V., Borsani O., Zipfel C., Macho A.P., Botella M.A. TTL proteins scaffold brassinosteroid signaling components at the plasma membrane to optimize signal transduction in Arabidopsis. The Plant Cell (2019) doi: 10.1105/tpc.19.00150. Link
Macho A.P.* & Lozano-Duran R.* Molecular dialogues between viruses and receptor-like kinases in plants. Molecular Plant Pathology (2019) doi: 10.1111/mpp.12812. (* Co-corresponding authors). Link
Lee E., Vanneste S., Pérez-Sancho J., Benitez-Fuente F., Strelau M., Macho A.P., Botella M.A., Friml J. and Rosado A. Ionic stress enhances ER-PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis. PNAS (2019). Link
Zheng X., Li X., Wang B., Cheng D., Li Y., Li W., Huang M., Tan X., Zhao G., Song B., Macho A.P., Chen H., and Xie C. A systematic screen of conserved R. solanacearum effectors reveals the role of RipAB, a nuclear-localized effector that suppresses immune responses in potato. Molecular Plant Pathology (2019) online early 2018, doi: 10.1111/mpp.12774. Link
Yu G., Xian L., Sang Y. and Macho A.P. Cautionary notes on the use of Agrobacterium-mediated transient expression upon SGT1 silencing in Nicotiana benthamiana. New Phytologist (2019) online early 2018, doi: 10.1111/nph.15601. Link
Wang Y., Li Y., Rosas-Diaz T., Caceres-Moreno C., Lozano-Duran R., and Macho A.P. The IMMUNE-ASSOCIATED NUCLEOTIDE-BINDING 9 protein is a regulator of basal immunity in Arabidopsis thaliana. Molecular Plant-Microbe Interactions (2019) Online early 2018. Link
2018
Rufián J.S., Lucia A., Rueda-Blanco J., Zumaquero A., Guevara C.M., Ortiz-Martin I., Ruiz-Aldea G., Macho A.P., Beuzon C.R. and Ruiz-Albert J. Suppression of HopZ effector-triggered plant immunity in a natural pathosystem. Frontiers in Plant Sciences (2018). Link
Zhao Y., Zhang Z., Gao J., Wang P., Hu T., Wang Z., Hou Y.J., Wan Y., Liu W., Xie S., Lu T., Xue L., Liu Y, Macho A.P., Tao W.A., Bressan R.A., and Zhu J.K. Arabidopsis Duodecuple Mutant of PYL ABA Receptors Reveals PYL Repression of ABA-Independent SnRK2 Activity. Cell Reports (2018) 23: 3340 - 3351.e5. Link
Wei Y., Caceres-Moreno C., Jimenez-Gongora T., Wang K., Sang Y., Lozano-Duran R. and Macho A.P. The Ralstonia solanacearum csp22 peptide, but not flagellin-derived peptides, is perceived by plants from the Solanaceae family. Plant Biotechnology Journal (2018), ,16:1349-1362. Link
2017
Wei Y. #, Sang Y. # and Macho A.P. The Ralstonia solanacearum type III effector RipAY is phosphorylated in plant cells to modulate its enzymatic activity. Frontiers in Plant Science (2017) Nov 7; 8: 1899. Link
Jiang G., Wei Z., Xu J., Chen H., Zhang Y., She X., Macho A.P., Ding W. and Liao B. Bacterial Wilt in China: history, current status and future perspectives. Frontiers in Plant Science (2017) Sep 11; 8: 1549. Link
Sun Y.#, Wang K.#, Caceres-Moreno C.#, Jia W., Chen A., Zhang H., Liu R.* and Macho A.P.* Genome sequencing and analysis of Ralstonia solanacearum phylotype I strains FJAT-91, FJAT-452 and FJAT-462 isolated from tomato, eggplant, and chilli pepper in China. Standards in Genomic Sciences (2017) 12:29. Link
Puigvert M., Guarischi-Sosa R., Zuluaga P., Coll N.S., Macho A.P., Setubal J.C. and Valls M. Transcriptomes of Ralstonia solanacearum during root colonization of Solanum commersonii. Frontiers in Plant Science (2017) 8: 370. Link
Sang Y.Y. & Macho A.P. Analysis of PAMP-triggered ROS Burst in Plant Immunity. Methods in Molecular Biology (2017) 1578:143-153. Link
2016
Sang Y.Y., Wang Y.R., Ni H., Cazalé-Noel A.C., She Y.M., Peeters N. and Macho A.P. The Ralstonia solanacearum type-III effector RipAY targets plant redox regulators to suppress immune responses. Molecular Plant Pathology (2016). Final publication 2018 Jan; 19 (1): 129-142. Link
Rosas-Díaz T., Macho A.P., Beuzón C.R., Lozano-Durán R. and Bejarano E.R. The C2 Protein from the Geminivirus Tomato Yellow Leaf Curl Sardinia Virus Decreases Sensitivity to Jasmonates and Suppresses Jasmonate-Mediated Defences. Plants (2016) 5 (1), 8. Link